UMA ABORDAGEM METODOLÓGICA PARA O ESTUDO DAS ZOOXANTELAS DE CORAIS DO BRASIL
DOI:
https://doi.org/10.18764/Resumo
Os procedimentos metodológicos necessários para o estudo das zooxantelas e de outros microssimbiontes hospedados por corais escleractínios do Brasil são apresentados neste trabalho. Incluem-se detalhes sobre técnicas de coleta, extração dos tecidos dos corais, estimativas das áreas das colônias do coral utilizado nos estudos quantitativos com zooxantelas, análises microscópicas das zooxantelas, análises dos pigmentos fotossintetizantes, estudo microscópico de outros microssimbiontes associados, além da caracterização genotípica das linhagens das zooxantelas de corais empregando-se técnicas de PCR, e sobre o monitoramento do branqueamento. Finalmente discutem-se aspectos chaves das metodologias que devem ser considerados a fim de garantir resultados seguros.
Palavras-Chaves: Zooxantelas, Dinoflagelados, Corais escleractínios, Brasil, Metodologia
Abstract
A methodological approach to study of the Zooxanthellae from Brazilian corals
The necessary methodological procedures for the study of the zooxanthellae and other microsymbionts of scleractinian corals of Brazil are presented in this study. Details of the collection techniques, extraction of the coral tissue, determination of the area of the coral colonies used in the quantitative studies on zooxanthellae, microscopic analyses of the zooxanthellae, analyses of the photosynthetic pigments, microscopic study of other associated microsymbionts, genotypic characterization of the zooxanthellae hosted by corals using molecular techniques, and the bleaching monitoring are included. Finally the key aspects of the methodologies that should be considered in order to guarantee valuable results are discussed.
Keywords: Zooxanthellae, Dinoflagellates, Scleractinian corals, Brazil, Methodology
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Referências
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